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GHK-Cu is endogenous to the human body (found naturally in plasma, saliva, and urine), and its concentration naturally declines with age

Samples (10 g in triplicate) were harvest on days 0, 1, 3, 5, and 7, washed twice with 50 mM TrisHCl (pH = 7)10 mM EDTA for 20 min and kept at 80C

Consequently, the second principle for fermenting mushrooms is to destabilize these enzymes via salting, or lactic acid producing bacteria and afterward the time of their storage can vary from 4 weeks up to 6 months (Jaboska-Ry et al., 2019a, 2019b, 2019c, 2019d)